Enzymatic Joining of Polynucleotides
نویسندگان
چکیده
منابع مشابه
Enzymatic Synthesis of Polynucleotides
Several publications from this laboratory have dealt in a preliminary fashion with the preparation and properties of highly purified polynucleotide phosphorylase from Azotobacter vinelan&i (2-4). A more detailed account of this work is presented in thii paper. Although polynucleotide phosphorylase preparations can catalyze the synthesis of a variety of homopolymers (polyadenylic acid, polyuridy...
متن کاملEnzymatic Synthesis of Polynucleotides
Several publications from this laboratory have dealt in a preliminary fashion with the preparation and properties of highly purified polynucleotide phosphorylase from Azotobacter vinelan&i (2-4). A more detailed account of this work is presented in thii paper. Although polynucleotide phosphorylase preparations can catalyze the synthesis of a variety of homopolymers (polyadenylic acid, polyuridy...
متن کاملEnzymatic Breakage and Joining of Deoxyribonucleic Acid
Polynucleotide ligase from Escherichia coli infected with bacteriophage T4 catalyzes the esterification of interrupted phosphodiester bonds (single strand breaks) within a DNA duplex. Purified preparations of the enzyme also catalyze an isotopic exchange reaction between ATP and 3’PPi. However, they do not catalyze a measurable exchange between 3H-AMP and ATP. A radioactively labeled ligaseAMP ...
متن کاملStudies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5'-end carries a phosphate group and the complementary deoxynucleo...
متن کاملEnzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and RNA polymerases in vitro. Polynucleotides containing low levels of biotin substitution (50 molecules or fewer per kilobase) have denaturation, ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)78188-5